[Objective] This experiment studies the genetic characteristics of Lactobacillus plantarum from different sources and its genetic diversity in different environments, and explores the similarities and differences in the adaptability of two strains of L. plantarum A8 and P9 in the animal intestine and plant surfaces, so as to provide a theoretical basis for the development of excellent strains.[Methods] This study analyzed the genomes of L. plantarum A8 and L. plantarum P9 isolated from the Animal intestines and plant surface. The research uses Next Generation Sequencing (NGS), based on the Illumina NovaSeq sequencing platform, and using the third-generation single-molecule sequencing technology, based on the PacBio Sequel sequencing platform to sequence L. plantarum A8 and L. plantarum P9. Carbohydrate-active enzymes (CAZy), Koyto encyclopedia of genes and genomes (KEGG), and Clusters of orthologous genes (COG) databases are used to annotate the genome function; CGView software is used to draw the genome circle map of the strain. At the same time, comparative genomics was used to compare with other published L. plantarum genomes.[Results] The research shows that the L. plantarum A8 and L. plantarum P9 genomes were different in genome size, the phylogenetic tree showed that the two strains were in the same branch with L. plantarum from other sources, and L. plantarum P9 was the closest to L. plantarum WLPL04 from breast milk, while L. plantarum A8 was the closest to L. paraplantarum DSM 10667. According to gene family analysis, there are 2643 common genes in the two strains, including some anti-stress proteins, such as heat shock proteins and cold shock proteins. There are 321 and 336 unique genes in plantarum A8 and P9, respectively. The unique genes in L. plantarum A8 are mainly involved in DNA replication, ABC transport system, PTS system, sulfonate transport system, amino acid biosynthesis and other metabolic pathways; The unique genes of L. plantarum P9 are mainly involved in carbohydrate transport and metabolism, such as rpiA gene, lacZ gene, fruA gene and so on.[Conclusion] By analyzing the genomic information of L. plantarum with comparative genomics method, it was found that L. plantarum from animal intestine has better amino acid transport ability, and the L. plantarum strain attached to the surface of the plant has good carbohydrate utilization ability, which provides a theoretical basis for the development and utilization of probiotics.