Abstract:［Objective］ We intended to obtain and characterize lactic acid bacteria with high capacity of cholesteroldegrading．［Methods］ We chose Jiangshui as the experimental material，screened lactic acid bacteria by the culture medium with high cholesterol，and studied other features of lactic acid bacteria like salt-tolerant，acid resistance，then identified the species of lactic acid bacteria by combining physiological and biochemical methods and 16S rDNA sequence．［Results］All lactic acid bacteria isolated had the capacity of cholesterol-degrading to some extent．There were 4 strains had high cholesterol-degrading rate (＞75%)．Four strains were Lactococcus lactis subsp． lactis，two were Brevibacterium casei，and one was Lactococcus raffinolactis．［Conclusion］Cholesterol-degrading lactic acid bacteria were screened from Jiangshui，with application potential for cholesterol degradation.