Abstract: 【Objective】To obtain new strains that can dissolving phosphate, we screened and identified a new strain Z32 capabile of phosphate dissolving from soil samples. We studied the colonization and phosphate-dissolving characteristics of Z32 in soil.【Methods】Morphological, growth on various media, and ITS rDNA sequences homology analysis were performed to identify the strain Z32. We conducted a soil experiment in pot to investigate the colonization and the capbility of the different forms of inorganic phosphorus transformation of the strain Z32.【Results】The strain Z32 was identified as Penicillium aculeatum. Z32 dissolved all Ca3(PO4)2 in solid culture for 4 days and decreased the pH from 7.0 to 1.5 in potato liquid culture for 18 hours. Z32 presented high colonization ability in soil and the optimum colonization temperature was 20℃. The number of Z32 increased 9.83 times in 21 days and kept alive in 49 days at the optimum temperature. Z32 promoted the transformation of insoluble phosphate like Ca8H2(PO4)6?5H2O, AlPO4 and FePO4 to soluble CaHPO4 and the CaHPO4 content increased by 58.83% in soil compared to CK at the 21st d at 20℃. Conclusion】 A new strain Z32 with high performance of phosphate dissolving had colonized well in soil. Z32 showed high phosphate-dissolving ability for different kinds of inorganic phosphorus in soil. At the same time, Z32 inhibited the transformation of CaHPO4 to insoluble phosphate.