[Objective] Methane oxidizing bacteria are important indicators for oil and gas exploration. For example, characteristics of methane-oxidizing bacteria can be used to predict deep oil and gas reservoir. [Methods] We studied the distribution of methane-oxidizing bacteria above typical reservoirs in Shunbei by using molecular biology techniques combined with geochemical hydrocarbon indices. [Results] The copy number of pmoA gene above the oil and gas field was positively correlated with the content of acid-hydrolyzed hydrocarbon, and the oil and gas region was 0.5-2 orders of magnitude higher than the background region. The results of high-throughput sequencing of 16S rRNA gene and cloning library of pmoA gene showed that the oil reservoir was mainly type I methane oxidizing bacteria in Shunbei, as the distance from the oil field increases, the type I to type II phenomenon of methane oxidizing bacteria exists. The abundance of Methylomonas sp. was quite different in the background area and the oil and gas area. [Conclusion] The characteristics of methane oxidizing bacteria during long-term micro-osmosis of hydrocarbons have certain indications for predicting deep reservoirs, Combining geological profiles and surface hydrocarbons can effectively predict favorable reservoir areas.