Microbiology, as a basic course in biology, plays a vital role in building students’ knowledge systems and teaching values in classroom. In order to achieve the goal of “golden class” construction, the microbiology course adheres to the solid foundation and pays attention to the cutting-edge concept in the construction process. It emphasizes the connection and transition of different courses in the curriculum designing, and provides special lectures. Besides, we built high-quality teaching materials, apply the platform of “duifene”, and carry out activities such as “I am the keynote speaker” to enhance the quality of teaching and extend the teaching effect. At the same time, we also have a deep understanding of talent training, not only to cultivate intelligence, but also to educate people, to combine professional content in teaching, to stimulate students’ sense of social responsibility and mission, to give students the goal and guidance of learning by role models, to strengthen moral education, and then achieve the goal of all-round education.