Porcine enteric coronavirus, an important agent of diarrhea in piglets, primarily infects the villous epithelia of the small intestine, causing substantial economic losses to the pork industry. The lack of an in vitro model that can recapitulate the highly complex physiological properties of the gastrointestinal tract significantly limits the study of the interactions between porcine enteric coronavirus infection and host intestinal epithelium. With the rapid development of stem cell technology, an in vitro model that can mimic diverse cellular nature and complex structure of the intestine-enteroids, has attracted widespread attention. Compared with conventional cell lines, enteroids not only simulate the structure and function of the intestine, but also retain the genetic characteristics of the host. Enteroids would be expected to be an ideal model for studying the interactions of host-enteric pathogens. This article reviews the recent research progress of porcine enteric coronavirus and the applications of enteroids in enteric pathogens research, in order to provide new insights for the future fundamental research of porcine enteric coronavirus.