Agricultural microbiology plays an important role in the sustainable development of modern agriculture. In order to keep pace with the profound innovation and promotion of the modern agricultural industry in China nowadays, high-tech specialists will be highly in demand. Therefore, the content of Microbiology course curriculum is renovated and optimized according to the feedback from the collaborating enterprises. An online/offline integrated mode has been adopted, both theoretical and experimental course have been integrated to reinforce students’ understanding and application of agricultural microbiology techniques. The renovated course will be more practical and straightforward to prepare current students for their future application in modern agricultural industry.