[Background] The preservation method and its effect are the premise and guarantee for the quality of strains. It is more and more urgent to select the best preservation method for strains of Hypsizygus marmoreus with the increasing of cultured scale and yield year by year. [Objective] Some common preservation methods and their effects were analyzed and studied in order to find a simple, efficient and inexpensive preservation method for strains of Hypsizygus marmoreus. [Methods] The preservation effect of each preservation method was evaluated by comparing the growth rate of mycelial, dehydrogenase activity of mycelial and the decolorization rate of mycelium to LBL medium. [Results] By comparing and analyzing the three-month preservation effects of three kinds, 27 different preservation methods of Hypsizygus marmoreus strains, it was found that the best preservation method was water solution preservation, among which 0.1% PEG6000 water solution was the best; the second was sawdust preservation method, among which the saline-soaked poplar sawdust was the best; The slant preservation method was the third. The effect of normal preservation experiment was better than that of accelerated preservation experiment. [Conclusion] Compared with the conventional preservation methods for strains of Hypsizygus marmoreus, the preservation methods adopted in this experiment greatly broadened the types and scope of the preservation methods, and improved the preservation effects of strains.