In order to explore the application effects of WPBL didactics in the education of “Microbiology Laboratory”. 66 students majoring in laboratory diagnostic medicine were respectively divided into two teaching groups. The WPBL didactics was carried out in the experimental group while the traditional didactics was used in the control group. Theoretical tests were performed to both groups and questionnaire was given to the experimental group at the end of the class. The statistical the experimental group test results clearly superior to the controls (P < 0.01), and the pass-level differences of theoretical test between two groups have statistical significance (P < 0.05); and over 90% of the students were interested in the WPBL didactics as they considered it effective to improve their learning interests, self-learning ability and problem-solving skills. From the result, WPBL didactics can enhance the teaching quality of “Microbiology Laboratory”.