Strain FDB, a fenvalerate-degrading bacterium, was isolated from some long-term pesticide-contaminated agricultural soils. The strain was identified as Pseudomnas aeruginosa based on its morphological and physiological properties, and 16S rDNA sequence analysis. Strain FDB grew in the medium supplemented with fenvalerate as its sole carbon source and degraded fenvalerate isomers at the concentration of 100 mg/L at the degradation rate from 69.06% (SR + RS) to 64.32% (SS + RR) in 5 days shaking at 30°C. The optimal growth was obtained under 35°C, pH 7.0 and 75 mL medium content 250 mL flask through shaking. The crude fenvalerate-degrading enzyme solution from cellular disruption was prepared by sonication, the activity of fenvalerate-degradation was associated with the intracellular fractions.