In this paper, by fermentation xylose in shake flask, two oleaginous yeasts named H2-1 and J2-2 which had high performance in utilizing sugar cane bagasse hemicellulose hydrolysates were selected from ten oleaginous yeasts isolated in our laboratory before. The lipid coefficient of two yeasts reached 13.49 and 10.23, respectively, shows a high efficiency transformation into lipid by utilizing sugar cane bagasse hemicellulose hydrolysates. H2-1 was identified as Rhodotorula minuta and J2-2 was identified as Rhodotorula glutinis based on physiological and biochemical characteristics and 26S rDNA sequence analysis.