Methanotrophs can use methane as the only carbon source and energy sourcey to effectively remove nitrogen in the oxidation, which can be divided into aerobic methane oxidation coupled to denitrification (AME-D) and anaerobic methane oxidation coupled to denitrification (ANME-D), and thus, they are of vital significance in the researches of carbon and nitrogen cycles. In this paper, after summarizing the classification and distribution of methanotrophs in recent years, the basic principles, influencing factors and application of AME-D and ANME-D are then expounded, and in the end, the related future trend is analyzed and proposed, in an attempt to make a slightest contribution to the studies of methanotrophs-based applications in wastewater denitrification.