With the rapid development of mobile network technology, its educational functions have been gradually explored, and show the prospect of good mobile learning applications. In this paper through building the learning resources base on “Zhejiang Online University APP” and “WeChat Public Platform”, we design blended teaching model based on mobile learning, and apply it to the teaching reform practice of Biochemistry courses. The results of classroom teaching practice and survey feedback show that applying mobile learning to mixed teaching is a popular teaching model with students and has a good teaching effect.