The twenty-first century is characterized by increasing cross-section of multiple disciplinary. This is more prominent in modern biology, with fundamental biological issues addressed by integrating multiple methodologies from diverse branches of science, including but not limited to biology. This presents new immense challenge for undergraduate education in the campus. To explore a solution to meet such challenge, based on our study, mycobacterial secretion system was used as an example to explore the philosophy and work flow of integrated teaching model in Microbiology and its advantages in undergraduate education.