[Objective] We studied resources, diversity and taxonomic information of the genus Hirsutella. [Methods] We identified two fungal samples GZUIFR-dj14 and GZUIFR-dy1 from Guizhou Province, which parasitized adult of Cercopidae (Homoptera) and Dioryctria splendidella (Lepidoptera) through morphological characteristics and phylogenic analyses based on tef1, ITS and 28S rDNA sequences. [Results] The two fungi were identified as Hirsutella changbeisanensis and Hirsutella leizhouensis. [Conclusion] Two new fungal isolates were studied with their information of hosts, habitats, molecular sequences and phylogenetics.