[Objective] To isolate and characterize the lytic phage from fecal samples of dairy cattle in Shihezi, Xinjiang. [Methods] The phage was isolated and purified from dairy cattle fecal samples by double-layer agar plate method. The purified phage was concentrated negatively stained with uranyl acetate and observed by transmission electron microscopy. Meanwhile, the genome of the isolated phage was sequenced. Its genetic and evolutional history were analyzed. In addition, the host range, optimal multiplicity of infection (MOI), one-step growth curve, temperature and pH stability of the phage were investigated. [Results] One strain of phage that lysed Escherichia coli was isolated and named vB_EcoM_XJ2. The phage produced circular, not clear plaques with 0.7 mm–1.2 mm diameter. The electron microscope observation showed that the phage had a symmetrical Polyhedra the head and a contractile tails. The genome of the phage was comprised of double strand DNA, and the size was 75.617 kb with G+C% content of 42.09%. The homology of amino acid sequence of vB_EcoM_XJ2 was 94% with Escherichia coli phage NJ01 and vB_EcoP_SU10. The isolated phage was lytic to many strains of Escherichia coli, isolated from bovine mastitis milk samples. The phage could withstand the temperature up to 60 °C and keep stable titer under pH 5.0–11.0. The optimal MOI was 0.1, the incubation period was 15 min, the burst phase time was 95 min, and the burst size was 10.6 PFU/cell. [Conclusion] The newly isolated phage, named vB_EcoM_XJ2, belongs to lytic phage of Myoviridae, which shows strong capacity to adapt different environments conditions such as temperatures and acid-base values.