[Objective] Multidrug-resistant strains of Enterococcus faecalis are becoming increasingly common worldwide. Therefore, we aimed to find novel agents to control antibiotic-resistant E. faecalis. We isolated a lytic bacteriophage from hospital sewage. [Methods] Phage morphology was observed using transmission electron microscopy (TEM), and its genome was sequenced using the Ion Torrent sequencing platform. Genome annotation and comparative and evolutionary analyses were performed following assembly of the complete genome sequence. [Results] Lytic bacteriophage vB_EfaP_IME195 was successfully isolated from infected E. faecalis host cells. TEM analysis indicated that the isolated bacteriophage resembled members of the Podoviridae family. The complete genome of vB_EfaP_IME195 was 18 607 bp long, and was comprised of circular dsDNA (GenBank accession No. KT932700). The genome had a G+C content of 33%, and contained only 27 coding sequences. BLASTn analysis showed that vB_EfaP_IME195 shared the highest homology (82%) with E. faecalis phage vB_Efae230P-4. [Conclusion] The isolation and characterization of this novel lytic E. faecalis phage provides the basis for potential treatment alternatives for multidrug-resistant E. faecalis infection.