As the important component of the biology, Microbiology is the basic course of some professions. Microbiology teaching followed the similar classical model which emphasized on the curriculum theory system in different universities. But this model isn’t conducive to the undergraduate’s application ability training in the ordinary universities. Therefore, to make basic course work at full capacity, the author optimized the teaching contents of theory and practice, and integrated the two complementarily. During optimizing, the author regarded the choosing and reordering of experimental project as breakthrough point. All the work based on the construction procedure of microbiological research thinking and practical application.