[Objective] The aim of this study is to isolate the arbuscular mycorrhizal fungi (AMF) from different ecological environments in China, and lay the foundation for the research of AMF. [Methods] Relying on sorghum as the host plant, AMF strains were isolated from the soil using the trap cultures, single species cultures and stimulation of sporulation technologies respectively, followed by the species identification. [Results] One hundred and thirty-five AMF strains belonging to 23 species were isolated from the rhizosphere soil of over 50 host plants in 45 regions of China, and their morphological characteristics were described. [Conclusion] China is rich in the arbuscular mycorrhizal fungi resource, and the species resource.