[Objective] Solid-state fermentation (SSF) media for growth of the two endophytic fungal strains (YCEF005 and YCEF053) were investigated. [Methods] The composition of fermentation substrates were optimized through single factor experiment and orthogonal experimental design. The additional nutrients and inoculum dose on the basis of the fermentation substrates were optimized through the combination of single factor experiment, uniform experimental design as well as artificial neural network with genetic algorithm. [Results] The optimal fermentation substrates for YCEF005 are as follows (W/W): 50% wheat bran, 10% bean cake powder, 20% rice bran, 20% corn flour. The optimal fermentation substrates for YCEF053 are as follows (W/W): 60% wheat bran, 10% bean cake powder, 10% rice bran, 20% corn flour. The optimal additional nutrients and inoculum dose (per kg substrates) for YCEF005 is as follows: sucrose 12.96 g, peptone 12.70 g, NH4NO3 4.00 g, KH2PO4 1.50 g, CaSO4 10.00 g, MgSO4 0.48 g, water content 500 g, inoculum dose 24 mL. The optimal additional nutrients and inoculum dose (per kg substrates) for YCEF053 is as follows: sucrose 13.37 g, peptone 14.02 g, NaNO3 3.85 g, KH2PO4 1.23 g, CaSO4 10.89 g, MgSO4 0.52 g, water content 480 g, inoculum dose 20 mL. The maximum fungal biomass was achieved when cultured on the optimal medium for 7–9 d. [Conclusion] The biomass of fungal SSF was improved by optimizing the media compositions.