In this paper, author explore the teaching pattern of microbiological class by teaching effects and evaluation system, based on classroom studying in Benedictine University for two months, as well as communicated and cooperated with microbiological professional teacher. The teaching mode emphasizes the cultivation of students’ comprehensive qualities, pay attention to the inspiration and interest. At the same time, theoretical teaching coordinates with practical teaching and extracurricular scientific activities. The research provides certain reference for microbiology teaching in China.