According to the specialty aim of normal university and the new curriculum reform of middle school, Research-oriented teaching mode has been performed in the Microbiology teaching. In the process of teaching, it emphasizes the students’ consciousness and abilities of Research-oriented teaching practice training by leading students to experience the teaching contents, teaching methods, research activities and some other aspects. At the same time, in the Microbiology teaching, it strengthens the teacher’s guiding role and the connection with the middle school biology teaching. The teaching practice showed that the Research-oriented teaching mode that combines the Microbiology and the biology in middle school, not only stimulated students’ interest in studying the course, promoted active thinking, but also developed their consciousness and abilities of Research-oriented teaching mode practicing. So it meets the demand of the new curriculum reform of middle school.