Due to the difficulty in doing the productive practice for students majoring in bio-pharmaceutical in higher vocational education because of the particularity of industry, on-campus simulating-enterprise model for productive practice was constructed to solve the problem. Through this model, the consistency of training and production was achieved; students’ vocational skills and occupational quality were improved, as well as the remarkable results have been obtained. There are mainly five contents in the model. The first is to form a teaching team with rich production management and teaching experience, the second is to develop project named “Polymyxin E Productive Practice” based on antibiotics production process followed by development of the productive practice textbooks, the third is to establish a higher simulating and open training base used for scientific research and social service, the fourth is to build the mode of enterprise-style production organization and management, and the last is to create a grading evaluation and procedural assessment system.