Small RNAs in bacteria is a kind of 40?400 nt non-coding RNAs which play an important role in regulation while the environmental conditions (such as temperature, nutrition, outer membrane protein, pH and iron) change. Small RNAs generally transport environmental stress signals and stress responses through the two component signal system and regulatory proteins, and play functions via antisense base pairing with target mRNAs to regulate translation and degradation of target mRNA, or via directly binding proteins and then modulating their activities. This paper reviewed the regulation roles and the response mechanism of sRNAs in bacteria in different stress.