The biofilms formed by bacteria can protect them from antibiotics, it also can bring difficulties for the treatment of infectious diseases and the sterilization of clinical machines. Recent research progress showed that bacteriophages and their lysins are potential use for eradication of biofilm, which is of great significance in control of various infectious diseases clinically. Bacteriophages can eradicate abiotic biofilms as well as zoetic ones. The degradation of biofilms caused by lysins such as LySMP, CHAPk and CWHs is probably associated with their activity of direct bacteriolysis and extracellular matrix degradation. In addition, the combined use of antibiotics, cobalt ion or chlorine with bacteriophage may have a better effect to biofilms. The review discussed the action of bacteriophages, their lysins and combination with other materials to eliminate biofilms and we hope they can be used clinically in the future.