To determine the structure, classification and functional aspects of G protein-coupled receptor (GPCR) in filamentous fungi, and to determine the relationship of GPCR between filamentous fungi and other model organisms. Based on the reported GPCR in the model organisms and filamentous fungi, the conserved domain was analyzed by SMART web service, and the genetic relationship between filamentous fungi and other model organisms was identified by Clustal X and MEGA software. There were seven transmembrane domains of typical GPCR and novel GPCR with PIPK, RGS and other conserved domains in filamentous fungi. The classification of GPCR from different scholars was identificated, as well as novel GPCR has special function. The model organisms GPCR and filamentous fungi GPCR can be grouped into each category. The number of GPCR of filamentous fungi was less than that of the model organism, and the number of GPCR was not the same among fungal taxa. GPCR in filamentous fungi contains a number of other conserved domains in addition to a typical seven transmembrane, which provide important theoretical basis for further study on its function.