[Objective] To obtain endophytes which has the plant-growth promote function from alfalfa. [Methods] Two strains were isolated and purified from fresh tissues of alfalfa, and then analyzed by 16s rRNA gene sequence, identified by the physiological and biochemical methods. The plant-growth promote properties to alfalfa of two strains were detected. [Results] Two alfalfa endophytes, ASR16 (Pantoea vagans) and ALR33 (Bacillus pumilus) were obtained. Both of the endophytes can produce IAA and siderophore, have phosphate solubilization activity, and can promote the growth of alfalfa effectively. [Conclusion] Two plant endophytes, ASR16 and ALR33, with plant-growth promotion to alfalfa could be used as the basis of bacterial manure research.