[Objective] The research is to study the effects of endophytic fungi re-inoculation on folia endogenous flora structure of grape. [Methods] Eight strains of endophytic fungi which isolated from wine grape leaves were re-inoculated to the healthy leaves of grapevine (variety: Rose-honey), and the effects on the folia endophytic fungi structure of grape were analyzed. Meanwhile, effects differences on the endophytic fungi structure to both groups of grapevine with and without pesticide after endophytic fungi re-inoculation, were also studied. We describe the folia endophytic fungi structure by using the isolation rate, dominance index and diversity index three months after re-inoculation. [Results] The results indicated that among all the treatments, five strains of the inoculated endophytic fungi (Xylaria sp., Nigrospora sp., Alternaria sp. 1, Alternaria sp. 2 and Colletotrichum sp.) can be relatively higher frequently isolated. Significant differences of folia endophytic fungi structures were found between one fungus re-inoculation and another. [Conclusion] Compared with the controls, fungi re-inoculation can increase the isolation rate, but decrease the diversity index significantly of folia endophytes. And the using of pesticide could decrease the isolation rate, but shares a higher diversity of folia endophytic fungi, compared with that of the pesticide free group.