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新疆低阶煤本源生物甲烷转化中微生物群落组成及变化
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新疆维吾尔自治区科技支撑计划项目(No. 201232121)


Microorganism community composition and changes during low rank coal authigenous microorganism methane coversion in Xinjiang
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    摘要:

    【目的】探究新疆低阶煤生物甲烷转化过程微生物群落组成及多样性。【方法】采用厌氧培养方法和末端限制性片段长度多态性技术(Terminal restriction fragment length polymorphism,T-RFLP)分析新疆低阶煤本源微生物对甲烷转化及有机酸含量的影响,分析新疆哈密大南湖长焰煤生物甲烷转化过程中微生物群落动态变化。【结果】研究表明长焰煤和褐煤对本源微生物产甲烷影响较小,随着低阶煤生物甲烷转化时间的延长,甲烷产量呈上升趋势,转化60 d后长焰煤甲烷产量高达10.28 mL/g,挥发性有机酸(VFA)浓度则最低;微生物多样性指数变化不明显,不同转化时间微生物主要类群为放线菌门(Actinobacteria),拟杆菌门(Bacteroidetes),厚壁菌门(Firmicutes),变形菌门(Proteobacteria);甲烷菌的群落结构相对于细菌较简单,在整个低阶煤生物转化产甲烷过程中共有古菌类群为甲烷八叠球菌属(Methanosarcina)、甲烷盐菌属(Methanohalobium)、甲烷叶菌属(Methanolobus)、甲烷食甲基菌属(Methanomethylovorans),它们是构成群落结构的基本菌群。【结论】低阶煤生物甲烷转化过程微生物群落具有丰富的多样性,且不同时期多样性有较大差异。甲烷菌群落结构相对于细菌较简单,共有类群明显。

    Abstract:

    [Objective] The composition and diversity of microorganism during low rank coal authigenous microorganism methane conversion were explored in Xinjiang. [Methods] We evaluated the effect of low rank coal authigenous microorganism on methane conversion and organic acid content by anaerobic culture. The dynamic changes of microbial community were analyzed by terminal restriction fragment length polymorphism (T-RFLP) in Hami’s long flame coal. [Results] The results suggested that lang flame coal and brown coal had little influence on methane production of indigenous microorganism. As low rank coal biological methane conversion time went on, methane production showed an increasing tendency. The yield of long flame coal biological methane was 10.28 mL/g and the concentration of volatile fatty acid (VFA) were the lowest at 60 days. The diversity indexes of microbes did not change significantly. The major microbial groups were Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria in different low rank coal conversion period. The bacterial community structure was more complex, while the methanogens community structure was relatively simple. All samples harbored these phyla such as Methanosarcina, Methanohalobium, Methanolobus, Methanomethlovorans, which constituted a basic flora of community structure. [Conclusion] There was abundant microbial diversity in different low rank coal conversion period and the diversity was different in different period. The methanogens community structure was relatively simple, and the number of common groups was higher in methanogens community.

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尤陆花,史应武,高雁,杨红梅,张涛,娄恺. 新疆低阶煤本源生物甲烷转化中微生物群落组成及变化[J]. 微生物学通报, 2014, 41(12): 2423-2431

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  • 在线发布日期: 2014-12-09
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