[Objective] Selecting biocontrol agents for preventing three kinds of soil-borne diseases (Fusarium oxysporum, Sclerotinia sclerotiorum and Rhizoctonia solani), promoting plant growth and clarifying their inhibitory efficiency as well. [Methods] Seventeen plant growth promoting rhizobacteria (PGPR) strains, acquired from preliminary studies, were used to test antagonism on pathogenic fungi by panel confrontation method and to measure the inhibition of mycelial growth by the PGPR strains. [Results] There were six strains efficiently antagonized Rhizoctonia solani. Among the six strains, the inhibition rate FX2 and LM4-3 was higher which could rise up to 73.82%. Seven strains efficiently antagonized Fusarium oxysporum, the inhibition rate of FX2 reached 66.81%. Four strains efficiently antagonized Sclerotinia sclerotiorum, the inhibition rate of strain LHS11 reached 85.71%. The strains of JM170 and LHS11 could prevent disease by secondary metabolites. All strains had a certain impact on mycelial growth of pathogenic fungi. [Conclusion] Strains LHS11 and FX2 that were screened had better biocontrol effects on three soil-borne fungous pathogen.