[Objective] It was studied that the effects of MIG1 gene and glucose on cell morphology of Saccharomycopsis fibuligera and the mechanism exploration. [Methods] S. fibuligera was cultured in YPD mediums with different concentrations of glucose and MIG1 gene knockout strain was in YPD medium. We studied the relationship between intracellular glucanase and chitinase activities and cell wall glucan and chitin contents and cell morphological changes. [Results] When the concentration of glucose in the media was lower, the mycelium of S. fibuligera decreased, single-cell yeast increased, glucanase and chitinase activities increased, and then glucan and chitin contents in cell walls reduced. The concentration of glucose had no significant effect on MIG1 gene knockout strain: glucanase and chitinase activities of MIG1 gene knockout strain were remained at a high level, glucan and chitin contents in cell walls was also lower, and most of cells existed as the form of single-cell yeast. [Conclusion] MIG1 gene and glucose by multicopy inhibitor of glucose regulated glucanase and chitinase activities which affected glucan and chitin contents in cell walls, so that the cell morphology of S. fibuligera changed.