[Objective] In order to estimate the application of some bacteria and yeast in the treatment of distillers’ grains. [Methods] Three strains of bacteria and 2 strains of yeast isolated from Luzhou flavor liquor brewing environment were mixed in different groups, then inoculated to the distillers’ grains by an inoculum of 2% without any additives. The main constitutions of the grains were detected to estimate the effects of these strains. [Results] The results showed that the content of acid and cellulose of the distillers’ grains decreased, and the protein increased in all treatments, especially, the cellulose in B5 degraded 23.89% and the content of the protein in B4 improved to 17.792% by a rate of 74.01%. [Conclusion] These mixed strains were predictively effective and available in lees feed or organic fertilizer producing.