[Objective] In order to obtain some thermo-tolerant strains of Lentinula edodes, the original strain 18 was mutated by UV induced protoplast mutagenesis. [Methods] The protoplasts of 18 were exposed to ultra-violet ray and the mutants were screened through the recover ability of mycelia after being treated at 47 °C for three hours. The growth rate of mycelia in sawdust medium during constant temperature process (25 °C), high temperature process (30 °C) and recover process (25 °C ) were determined. And the fruiting bodies of the mutants were collected at 25 °C. [Results] Fifty-seven thermo-tolerant mutant strains of 18 were obtained, and N6, N44 and N24 got good comprehensive properties. The growth rate during constant temperature process, high temperature process and recover process had correlation with fruiting characteristics. The growth rate during the recover process had positive correlation with fruiting production, single mushroom traits and high temperature tolerance capability, and it was selected as the predictor of integrated traits of thermo-tolerant mutant strains. [Conclusion] Basing on UV induced protoplast mutagenesis, some new thermo-tolerant mutant strains of Lentinula edodes were obtained.