In order to improve the microbiological experiment skill of the students, through experimental teaching research, the microbiology experimental teaching in higher vocational education reform discussion. Experimental design of logical coherence, strengthening basic experiment teaching and the creation of comprehensive experiments enhance experimental skills training. Creative experimental teaching methods, using multimedia displays, enhance practical ability of speaking practice fusion. Implementation of fitness exercises, strengthen the experimental process guidance and strictly regulate the operation, establish a better comprehensive assessment mechanisms to mobilize students' enthusiasm and initiative of the experiment to enhance experimental skills upgrading and improve the quality of teaching.