For the purpose of improving microbiology teaching effect, old concepts and methods should be abolished, good teaching habits and ethos should be established. In this paper, our practice in microbiology teaching under the complete credit system in four aspects including teaching and educating, circumstance designing, creative thinking and practice strengthening were introduced sequentially. It was indicated that our efforts in teaching reform had significant effect on microbiology study, and also could meet with the requirement for training high quality talents in biological science and technology in the new era.