The beta-cypermethrin degradation conditions including inoculum amount, temperature, pH, liquid volume, and initial concentration by Streptomyces HP-S-01 were systematically investigated. The results showed that Streptomyces HP-S-01 degraded beta-cypermethrin rapidly with a degradation rate up to 96% within 3 d, under the conditions of inoculum amount 0.6 g/L, 28 °C, pH 7.5, and liquid volume 50 mL/250 mL. The strain also could effectively degrade beta-cyfluthrin, beta-cyhalothrin, d-phenothrin and tetramethrin. Furthermore, the degradation reaction followed first-order kinetics and half lives (T1/2) were 0.78, 0.88, 1.08 and 1.24 d, respectively. The Andrews model was used to describe the beta-cypermethrin degradation process. The degradation kinetic data collected fitted the model well. The calculated parameters of qmax, Ks and Ki for the model were 1.826 3 d?1, 58.951 3 mg/L and 359.378 2 mg/L, respectively. And the optimal concentration of initial beta-cypermethrin was 145.553 5 mg/L.