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一株来自蚯蚓的产纤溶酶蜡状芽孢杆菌Bacillus cereus的筛选及鉴定
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贵州省科技厅创新能力建设项目[No. 黔科合G(2010)4009]


Screening and identification of a Bacillus cereus strain producing fibrinolytic enzyme from earthworm
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    摘要:

    对来自4个不同省份的5条蚯蚓的肠道及体表细菌进行分离, 共获得122株细菌。通过脱脂奶粉平板法初筛, 纤维蛋白平板法复筛, 以透明圈为筛选标记, 共筛选出产纤溶酶菌株12株, 其中菌株SC-3-W-3的纤溶酶活力较高, 达到了538.64 U/mL (相当于尿激酶的活力单位)。通过对其形态、培养、生理生化特征进行研究, 发现其与蜡状芽孢杆菌Bacillus cereus Frankland的特征很相符。进一步对SC-3-W-3的16S rDNA序列及系统发育分析表明, 该菌株与蜡状芽孢杆菌的同源性高达100%。综合生理生化及16S rDNA序列比对结果, 将SC-3-W-3菌株鉴定为蜡状芽孢杆菌。

    Abstract:

    One hundred and twenty-two bacteria strains were isolated from the intestinal tracts and body surface of five earthworms collected from four different provinces. Twelve fibrinolytic enzyme producing strains were screened based on the size of halo zone by using powdered skim milk and fibrous protein as the first and second round screening substrates, respectively. SC-3-W-3 strain with high fibrinolytic enzyme activity was identified as Bacillus cereus based on physiological and biochemical characteristics, as well as 16S rDNA. The fibrinolytic enzyme activity of SC-3-W-3 strain was up to 538.64 U/mL.

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张雪琴,康冀川,文庭池,何劲,雷帮星. 一株来自蚯蚓的产纤溶酶蜡状芽孢杆菌Bacillus cereus的筛选及鉴定[J]. 微生物学通报, 2011, 38(6): 895-902

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