In order to study the effects of endophytically fungal extracts and plant growth regulators on soybean rhizosphere bacterial diversity, the dynamics of rhizosphere bacterial community in different developmental stages was studied based on PCR-DGGE approach. The results showed that they could increase some bacteria in populations; but the variation of rhizosphere bacterial diversity by fermentation extracts and plant growth regulator was no obvious, and the growth stages as an important factor that affects the rhizosphere bacteria. In addition, we found that the advantage bacteria mainly is Proteobacteria, Acidobacteria, Nitrospira, Bradyrhizobium, etc. These are the common bacterial species in the root of soybean.