In this communication, it was discussed how to nurture undergraduate students’ entrepreneurial abilities during engineering microbiology teaching. Through the reform of examination methods, students were guided to design and write feasible reports for microbiological products to simulate entrepreneurship; the advanced researches in microbiology were introduced to cultivate students’ creation and innovation abilities; the national standard was taught to enhance their law consciousness and improve their insight on market; the economic cost was calculated to improve their financial management skill. This innovated teaching method would stimulate students’ enthusiasm for entrepreneurship and also cultivate their innovation and entrepreneurial abilities.