CLA (Conjugated linoleic acid, CLA) possesses a wide range of biological activities including anti-cancer activity, anti-atherosclerosis activity, capability of helping reduce weight fat and regulate immune system. The fatty acid isomerase from bacteria, which catalyzes the isomerization of linoleic acid (LA) to CLA production, is a promising candidate for other approachs. This paper introduces the source of linoleate isomerase, the mechanism of its function as well as reviewing recent advances re-garding the key property of this important enzyme. The preparation of linoleate isomerase and how to use it to produce high yield of isomers of conjugated linoleic acid with high purity are also demonstrated, which will help to realize sustainability and industrial scale production of conjugated linoleic acid.