Efficient storage of the wild germplasm is prerequisite for conservation and utilization of Termitomyces. In our study, using 5 isolated Termitomyces strains as materials, the preservation effects of distilled water preservation method and ?80°C freezing storage method were compared. These five wild strains of Termitomyces were stored in distilled water at room temperature and 4°C refrigerator, as well as preserved at ?80°C freezer for about 20 months after programmable cooler and styrofoam box controlled cooling. The survival rates of strains in distilled water were 100% after 20 months storage at room temperature, and that for frozen at ?80°C were 56%?76%. The strains preserved in distilled water at room temperature germinated in 4 to 10 d after inoculation, which is quicker than in ?80°C preservation (7?16 d). Styrofoam box method is an easy and effective method to control cooling speed compared to the programming method. In summary, preserving strains in distilled water at room temperature is a simple, practical and cheap method for long-term storage of Termitomyces germplasm.