Strain DA09202 with nematicidal activity was isolated from soil sample collected from Hainan Tropical Botanical Garden. Based on the mycelium morphology, physiological property, biochemical property and 16S rDNA phylogenetic tree, strain DA09202 was identified as streptomyces aureus. The results indicated that the strain DA09202 has nematicidal activity, the active compounds A23-1 and A46-2 were purified from its fermentation broth by means of solvent extraction, column chromatography, Sephadex LH-20 and preparation TLC, and the structure of the A23-1 was elucidated as 4￠,7-Dihydroxyisoflavone by spectrum analysis, and the structure of A46-2 is been elucidating.