Methanotrophs are a kind of bacteria which can use methane as the sole carbon source and energy for their anabolism and catabolism. A strain methanotroph was isolated from different samples of the Zoige Plateau, named XN1, and was studied. It was identified a strain of Methylomonas through 16S rRNA sequencing and comparison. The investigation result showed that the optimal conditions for the strain were methane gas together with methanol as carbon source and the coexistence of NH4Cl and KNO3 as nitrogen source, the cultivation temperature of 25°C and medium initial pH of 6.5, and the optional concentrition of CuSO4·5H2O of 0.03 mg/L and FeSO4·7H2O 0.4 mg/L.