Biochemistry is a fundamental and advanced subject in the field of life science. It’s also a course with difficulties in both teaching and learning. These features make it necessary for teachers to ceaselessly update teaching concepts, explore and create new teaching methods and means. Practice shows that satisfactory in-class teaching efficiency of this course can be achieved by flexibly adopting frameworks and icons, PBL methodology, comparative methodology, CBS methodology, and metaphorical teaching methods. Meanwhile, the integrated use of multimedia, bilingual education and other teaching methods also contributes to the improvement of the in-class teaching efficiency. With the help of the above teaching methods and means, abstract and difficult knowledge will become definite and easy. Students can also systematically master theoretical knowledge.