At the present time, probiotics used in recovering sea culture wastewater has some defects, such as no good counter, adaptation and relationship of these strains, so the applying effect is not satisfying. In our experiment, nitrosobacteria ZW38, denitrobacteria ZL5 and secreting proteinase strain ZS7, which were isolated from wastewater of marine culture, were mixed according the nitrogen cycle of seawater culture, the number of various strain and the content of NH4-N, NO2-N were determined. The results showed that these strains grow well each other when the ratio of ZW38, ZL5 and ZS7 is 3:2:1, and the total inoculating numer is 109 CFU/L. After 5 d for treating, the decomposed rate of resoluble protein and NH4-N is 94.0% and 77.5% respectively.