Screening seven strains of efficient phenol degradation bacterium in activated sludge from Harbin Taiping sewage treatment plant. These strains can use phenol as the only available carbon sources and energy. Based on inspects the growth and degradation of phenol in different temperature, pH value, and different phenol concentration. We can determine that the optimal temperature of seven bacterias is 10oC, the optimal pH value is 7.5, the lagest concentration of degradation of phenol is 3000 mg/L. From the study of degradation performance by these seven bacteriums shows that it has strong ability of phenol degradation. In the condition of 10°C, pH 7.5, fluid for 50 mL, vaccination is 15%, vibration velocity wave 160 r/min, degradation rate of 500 mg/L phenol can reach more than 90% after 48 h in the training. Glucose to the growth of bacteria and phenol degradation ability all have certain effects, when glucose concentration is 500 mg/L, the degradation rate of phenol is still more than 80%. The study on treatment contain other carbon sources of hydroxyl benzene wastewater has certain significance. Through the analysis DGGE strip map shows that the brightness can explain these bacteria are advantage bacterium in each system, and strong activityin sewage, its dominant position can be exist steadily. Rich strips are 2, 4, 24, 28, and it is showing its diversity in sewage system.