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毛壳霉CQ31的鉴定及固体发酵产木聚糖酶条件的优化
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国家自然科学基金资助项目(No. 30500099); 高等学校全国优秀博士学位论文作者专项资金(No. 200555)


Identification of Chaetomium sp. CQ31 and Optimization of Xylanase Production in Solid State Fermentation
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    摘要:

    从土壤中筛选出一株产木聚糖酶的真菌CQ31, 经鉴定后命名为毛壳霉CQ31。该菌能够利用几种农业废弃物固体发酵高产木聚糖酶, 玉米杆为最佳碳源。单因素优化试验表明: 以玉米杆为碳源, 胰蛋白胨为氮源, 初始水分含量80%, 初始pH值9.0为最佳产酶条件。在优化后的条件下培养7 d产木聚糖酶水平高达4897 U/g干基碳源, 此时甘露聚糖酶酶活达803 U/g干基碳源。因此, 毛壳霉CQ31固体发酵产木聚糖酶和甘露聚糖酶具有一定的工业化应用前景。

    Abstract:

    A fungus CQ31 isolated from soil samples was identified as Chaetomium sp.. This strain produced effectively xylanases utilizing several liguocellulosic materials in the solid-state fermentation (SSF), and corn straw was the best carbon source. The results of single-factor-experiment showed that the corn straw as the carbon source, tryptone as the nitrogen source, initial moisture content of 80% and initial pH 9.0 were the optimal conditions for xylanase production. Under the optimized conditions, it produced xylanase which was 4897 U/g dry substrate while mannanase was 803 U/g dry substrate after 7 days of cultivation. Therefore, xylanase and mannanase production by Chaetomium sp. CQ31 in SSF possess potential for commercial applications.

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丛倩千,江正强,吕顺意. 毛壳霉CQ31的鉴定及固体发酵产木聚糖酶条件的优化[J]. 微生物学通报, 2009, 36(8): 1269-1274

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