Strain 13-1 was gram negative biocontrol bacterial strains isolated from Amorphophallus konjac. In vitro antagonistic assay showed that strain could suppress the growth of seven plant bacterial pathogens and eight pant fungal pathogens. The results of phenotypic and general physiological and biochemical properties showed that strain 13-1 homologized with which descripted for genus Lysobcter. 16S rDNA determination and analysis was used for further identification, which showed that 16S rDNA sequence of strain 13-1 shared 99% homologies with published sequence of L.antibioticus DSM2044 from GenBank, and both sequences constituted a branch in phylogenetic tree. Based on these results, it is considered that the strain 13-1 belongs to one strain of L.antibioticus.