The reform and practice on teaching approaches of microbiology integrated case-based study, heuristic teaching, interaction teaching, comparison expression teaching and inductive learning was explored, which was contraposed the education aims of independent college and student’s trait, and combined to the fact of University of Electronic Science and Technology of China Zhongshan Institute. The result of practice showed that student enthusiasm for study was mobilized, and better results in teaching were got by application of diversiform teaching approaches.