To develop Students’ Practical Ability according to the teaching requirement and culture aim of preventive medicine major, the teaching plan, teaching content, teaching methods, and experimental checking methods were explored and the experimental teaching pattern of medical microbiology adapted to preventive medicine major was constructed. The investigation showed that the experimental teaching pattern helped to cultivate the students’ operating ability, thinking of scientific research and ability of aggregate and solving analysis. Moreover, it helped to develop the students’ co-operative consciousness and team spirit. It indicated that the new pattern was superior to the traditional experimental teaching.